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CRISPR guide templates
a benzoyl (Bz) base-protecting group on adenine
Synthetic RNA oligonucleotides
and calibration of nucleotide quantification assays
Stefania Cresti
DMT-2'-O-Me-G(dmf)-CE Phosphoramidite Amount/Package:10g / 240mL screwed bottle CRISPR guide templatesDMT 2' O Me G(dmf) CE Phosphoramidite is a 2' OMe phosphoramidite monomer used for solid phase oligonucleotide synthesis. It contains a 5' dimethoxytrityl (DMT) protecting group for 5' hydroxyl protection, a dimethylformamidyl (dmf) base protecting group on guanine, a cyanoethyl (CE) protecting group on phosphite, and a 2' O methyl (2' OMe) ribose modification The 2' OMe modification improves duplex stability, reduces immunogenicity, and significantly
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