UniOne® TR-FRET Human BCL6/CRBN PROTAC Binding Kit Size:2500T and other conditions arising from

and other conditions arising from compromised oxidative phosphorylation and cellular energy failure

as well as higher alkaline phosphatase (ALP) activity and extracellular calcium deposition

checked by color reaction Low OD value Incorrect plate reader settings Check instrument wavelength No stop solution added Add an appropriate amount of stop solution Wait time too long when reading the board Timely plate reading Excessive sample content The appropriate dilution factor was determined by pre-experiment Sample content is too low The appropriate dilution factor was determined by pre-experiment Background height Contamination of chromogenic solution Change the color developing solution Color development time is too long Controlling color development time Wrong dilution of detection antibody or enzyme conjugate Use recommended dilution method Incomplete plate washing Sufficient number of washes and addition of sufficient amount of washing liquid Theory This kit uses double-antibody sandwich enzyme-linked immunosorbent assay (ELISA)

thereby releasing cytochrome c and other apoptogenic factors into the cytosol to trigger the caspase cascade and ultimately apoptosis

Expression System HEK293 Molecular Weight Predicted MW: 60

UniOne® TR-FRET Human BCL6/CRBN PROTAC Binding Kit Size:2500T and other conditions arising fromProduct Specification Host Human Stability & Storage Store the kit at 80C or below. When storedappropriately, the reagents remain stable until the expiration date marked onthe label. Once thawed, BCL6 and CRBN stock solutions can undergo oneadditional freeze thaw cycle; labeled antibody solutions can undergo oneadditional freeze thaw cycle. To avoid repeated freeze thaw cycles, it isrecommended to aliquot the remaining stock solutions into single use