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Resuspend the cell pellet with an appropriate volume of DMEM complete medium and culture it in a cell culture dish
Add 2 μL Calibrator/Sample + 4 μL R2* + 4 μL R1 + 10 μL R3
one cognate receptor (IL-36R) and one accessory protein (IL-1RAcP)
enabling “trans-signaling” that markedly amplifies IL-6’s pleiotropic effects on growth
Staining PI staining working solution configuration: Add 25μl PI staining solution (solution A) and 10μl RNase A (solution B) to 0
7-DHC/DHCR7 Recombinant Rabbit mAb (S-3625-32) Other Proteins Resuspend the cell pellet withProduct Specification Host Rabbit Antigen 7 DHC DHCR7 Synonyms 7 dehydrocholesterol reductase; Cholesterol 5,6 epoxide hydrolase subunit DHCR7; Sterol Delta (7) reductase; Dhcr7 Immunogen Synthetic Peptide Location Endoplasmic reticulum Accession O88455 Clone Number S 3625 32 Antibody Type Recombinant mAb Isotype IgG Application WB Reactivity Ms, Rt Positive Sample Mouse testis, Mouse brain, Rat testis, Rat brain Purification Protein A Concentration 2
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