UniOne® TR-FRET Human IL21/IL21R Binding Kit Size:2500T Incubate the reaction at room

Incubate the reaction at room temperature (22–25°C) for 40 minutes

and HRP conjugate are sequentially added to microwells pre-coated with a Transcription Factor A

PD-1 can interact with its ligands PD-L1 (B7-H1) and PD-L2 (B7-DC) in a ratio of 1: 1

selectselect ①interface

Expression System HEK293 Molecular Weight 30-40kDa (Reducing) Purity > 95% by SDS-PAGE & SEC-HPLC Endotoxin <1EU/μg Conjugation Biotin Tag Avi Tag

UniOne® TR-FRET Human IL21/IL21R Binding Kit Size:2500T Incubate the reaction at roomProduct Specification Host Human Stability & Storage 80 Background This kit utilizes Homogeneous Time Resolved Fluorescence(TR FRET) technology to measure the interaction between IL21 and IL21R. Thismethod enables simple and rapid high throughput screening of inhibitors andantibody blockers. As illustrated, the interaction between IL21 and IL21R isdetected using an Eu labeled anti Tag1 antibody (TR FRET donor) and anAcceptor labeled anti Tag2 antibody